Holographic pixel superresolution in portable lensless on. A 3d printable platform for lensless holographic cellular imaging with open accessible software solutions has been developed delivering spatial resolutions of 1. Partially coherent lensless holographic microscopy with. In a regular optical microscope, the seen image is a highly magnified photographic image.
Digital inline holography dihm is an imaging modality within the fast. Color imaging in lensless digital holographic microscopy. Holoscopy makes ultrafast lensless imaging of scattering tissues possible. Osa lensless computational imaging through deep learning. Tiziani 2002 shortcoherence digital microcopy by use of lensless holographic imaging system, appl. The principles and some applications of the digital lensless holographic. Lensless singleexposure 3d imaging nick antipa, grace kuo, reinhard heckel, ben mildenhall, emrah bostan, ren ng, and laura waller. Tiny lensless endoscope captures 3d images of objects. Software algorithms are written to automatically identify the nematode eggs from low resolution holographic videos or images captured from a scanner. Improving lensless computational microscopy technology. Spotlight stock quote phase holographic imaging phi. In the literature, there is a significant number of approaches developed upon the traditional holography principle, where a splitter is commonly used to separate object and reference beams 41.
We experimentally built and tested a lensless imaging system where a dnn was trained to recover phase objects. An introduction to lensless digital holographic microscopy. Dlhm is a lensless methodology of imaging in two stages. Lensless inline dhm is the simplest arrangement, requiring no lenses, no mirrors, and typically only a light source, sample, and a digital imager chip. Basic lensless imaging for lowcost microscopy hackaday. Depth resolved imaging using multiangle lensless holography. After traveling a distance r, the phase of the reference beam in the plane of the. The innovative new technology takes data from ct and mri scans and transforms it into 3d holographic images using 3d glasses, a laser stylus, and a special display. In this paper, an irregular displacementbased lensless widefield microscopy imaging platform is presented by combining digital inline holography and. Our lensless system consists solely of a diffuser placed in front of a standard image sensor. In the color lihm, the sample is illuminated by sunlight, and the inline hologram is recorded by a color cmos imaging sensor located behind the sample. Judy yee, professor and vice chair of radiology and biomedical imaging and chief of radiology at sfvamc, described echopixels true 3d software. A lensless microscope capable of generating holograms of biotargets with. The lensless cellmicroscope combines holographic imaging of cells with a thermoelectric cooling system and automated cell segmentation for live cell imaging inside the incubator.
The presented 3dprintable portable opensource platform represents a fullyopen design, lowcost modular and versatile imagingsolution for use in high and lowresourceareasoftheworld. Osa multiangle lensless digital holography for depth. Pedrini, w osten 2005 application of shortcoherence digital holography in microscopy. Pdf lensless multispectral digital inline holographic microscope. Fast 3d imaging with lensless holographic endoscopy employing coherent fiber bundles, by juergen w. Osa color lensless inline holographic microscope with. Computational microscopy, sensing and diagnostics for telemedicine and global health applications. Project basic lensless imaging for lowcost microscopy. In this paper, an irregular displacementbased lensless widefield microscopy imaging platform is presented by combining digital inline holography and computational pixel superresolution using.
Digital lensless holographic microscopy and applications. Lensless microscopy of cell cultures 2014 wiley analytical science. Science foundation nsf cbet division biophotonics program, the nsf emerging frontiers in. Introduction digital inline holography dihm is an imaging modality within the fast evolving. Lensless microscopes, just like holographic ones, have several advantages. Interfacing the device with koala software from lynceetec is considered in. Digital holographic microscopy dhm is a technique that uses optical interference patterns to record a threedimensional optical field for imaging, sensing, and microscopy applications. Ozcan, partiallycoherent lensless holographic microscopy for automated semen analysis on a chip. Compact lensless digital holography infoscience epfl. The lensless cellmicroscope combines holographic imaging of cells with. The principle of holographic imaging invented many decades ago has significantly advanced since the original digital inline holographic experiment 2,40. Using holography instead of optical focusing enables to build a costefficient, robust and compact microscope.
The software also supports 3d imaging from the reconstructed stack of. The syringe pump was turned on at a flow rate of 1 ml per hour. The dispensing needle of the syringe was connected to the input port of the microfluidic flow chip. Deep learning has been proven to yield reliably generalizable solutions to numerous classification and decision tasks. Ozcan and mcleod, lensless imaging and sensing, 2016.
After sample cleaning using 50% optiprep by volume, a 1 ml solution of the interface layer was loaded into a syringe. Biomedical optical phase microscopy and nanoscopy, elsevier published in 20 isbn. The ozcan research group refereed journal publications. Czarske, elias scharf, and robert kuschmierz, will be presented monday, 16. Digital holography systems that employ shadow imaging projection of 2d. A 3d printable platform for lensless holographic cellular imaging with open. The aim of the present work is to develop digital dynamic holography as a metrological tool for both transmission and reflection applications. Holographic imaging and iterative phase optimization. Holopy digital holography and light scattering tools in python.
We propose a method for acquiring color images with a lensless inline holographic microscope lihm using sunlight illumination, which is suitable for weakly scattered amplitude objects. The details of the depth resolved imaging process using multiangle lensfree holography is summarized in fig. Portable lensless widefield microscopy imaging platform based on. But, if the object of study is transparent like, for instance, many living cells, photographic images cant be captured unless one uses special coloring agents. Both printer and reconstruction software source codes are open. Holograms can also be used for integral imagingbased 3d display, lensless projection, and lidar beam steering.
Dear colleagues, recent advancements in the semiconductor industry have inspired a revolution in compact sensors and computational platforms, creating a paradigm shift from lensbased imaging and sensing to computational imaging and sensing. This incoherent holographic imaging and diagnostic modality has orders of. Micromanager is an open source platform for controlling microscope hardware, automating image acquisition, and generating metadata about how images are acquired. The cooling of the cmos camera defines the local temperature of the integrated cell culture chamber. After the capture of the multiangle lensfree holographic images of the sample volume as illustrated in fig. By combining digital holography with optical coherence tomography oct in a process known as holoscopy, 3d parallel image acquisition with extremely high data throughput at high imaging speeds is possible due to the lack of moving elements. We demonstrate a compact and easytobuild computational camera for singleshot 3d imaging. Automated image analysis systems to quantify physical and.
A new lensless imaging system is built to record holographic videos of soil particles flowing through microscale flow assays. In this platform, lensfree digital holograms of the microobjects on the chip are recorded at different illumination angles using partially coherent illumination. In conclusion, we reported a lensless holographic superresolution microscope which is quite robust, easy to use, portable 95 g and costeffective, achieving a resolution of software platform converts existing 2d medical imaging data such as mri and. Continuous livecell culture imaging and singlecell tracking by computational lensfree led microscopy, sensors 2019. Here, we demonstrate for the first time to our knowledge that deep neural networks dnns can be trained to solve endtoend inverse problems in computational imaging.